Search results for "cigarette smoke extract"

showing 2 items of 2 documents

Oxidative stress and innate immunity responses in cigarette smoke stimulated nasal epithelial cells

2013

Cigarette smoke extracts (CSE) may play a significant role in diseases of the upper airway including chronic rhinosinusitis. Even short term exposure of cigarette smoke has adverse effects on mitochondrial functions and redox homeostasis in tissues which may progress to further complications associated with chronic smoking. Cigarette smoke alters toll-like receptor 4 (TLR4) expression and activation in bronchial epithelial cells. Carbocysteine is an anti-oxidant and mucolytic agent. The effects of carbocysteine on CSE induced oxidative stress and on associated innate immune and inflammatory responses in nasal epithelial cells are largely unknown. The present study was aimed to assess in CSE…

LipopolysaccharidesNecrosisNeutrophilsPhalloidineCARB CSE Cigarette smoke LPS Nasal epithelial cells ROS Reactive oxygen species TLR4 carbocysteine cigarette smoke extracts lipolysaccharide reactive oxygen species toll like receptor 4Fluorescent Antibody TechniqueApoptosisMucous membrane of noseCell SeparationBiologyToxicologymedicine.disease_causeCell LineNecrosisSmokeTobaccomedicineHumansExpectorantschemistry.chemical_classificationReactive oxygen speciesInnate immune systemCarbocysteineEpithelial CellsCarbocysteineTobacco ProductsGeneral MedicineActinsImmunity InnateToll-Like Receptor 4Nasal MucosaOxidative StresschemistryApoptosisImmunologyTLR4medicine.symptomReactive Oxygen SpeciesOxidative stressToxicology in Vitro
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Electrochemical Quantification of H2O2 Released by Airway Cells Growing in Different Culture Media

2022

Quantification of oxidative stress is a challenging task that can help in monitoring chronic inflammatory respiratory airway diseases. Different studies can be found in the literature regarding the development of electrochemical sensors for H2O2 in cell culture medium to quantify oxidative stress. However, there are very limited data regarding the impact of the cell culture medium on the electrochemical quantification of H2O2. In this work, we studied the effect of different media (RPMI, MEM, DMEM, Ham’s F12 and BEGM/DMEM) on the electrochemical quantification of H2O2. The used electrode is based on reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) and was obtained by co-electrode…

Mechanical EngineeringH2O2H<sub>2</sub>O<sub>2</sub>; electrochemical sensor; cell culture media; graphene oxide; gold; bronchial epithelial cell; lung adenocarcinoma cell; oxidative stress; cigarette smoke extract; resveratrolelectrochemical sensorgoldresveratrollung adenocarcinoma cellSettore ING-IND/23 - Chimica Fisica ApplicataControl and Systems Engineeringcell culture mediabronchial epithelial cellSettore ING-IND/17 - Impianti Industriali Meccanicigraphene oxideoxidative stressElectrical and Electronic Engineeringcigarette smoke extract
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